Chromatography Techniques in Organic Chemistry

Paper Summary:

This paper presents an experiment in which two types of chromatography were used to separate a mixture of two dyes, methylene blue and fluorescein. The materials and methods that were used for the experiment are discusses as well as the results that were obtained. The results are presented in a table form.

Table of Contents:
Introduction
Materials and Methods
Results
Discussion
Additional Experiments
Appendix

From the Paper:

"Gel permeation chromatography is a type of size exclusion chromatography (SEC), where the mobile phase is an organic solvent and separation occurs based on size. (Davies, Johnson, 2007). It is used to determine the molecular weights of organic and biological molecules, such as nucleic acids and proteins. The stationary phase is a gel medium, (e.g. agarose, polyacrylamide, or dextran) and elution occurs under low pressure. The molecules of interest are often further separated based on charge, acidity/bascitiy and affinity to particular molecules. This technique is efficient because all the samples can be simultaneously loaded without deterring the elution process, or disrupting the biologically-active state of the different samples. (Davies, Johnson, 2007)."

Sample of Sources Used:

• Bonicamp, Judith M., and Elizabeth B. Moll. "Column Chromatography of Dyes: Microanalysis with a Thin Layer Chromatography Scanner Works to Beat the Bands". Microchemical Journal, 55.2 (1997): 145-50.
• "Chemical Structure." ChemIDplus Advanced. 2005. U.S. National Library of Medicine. 28 Oct 2007. <http://chem.sis.nlm.nih.gov/chemidplus>
• Davies, Don R., and Johnson, Todd M. "Isolation of Three Components from Spearmint Oil". Journal of Chemical Education, 84.2 (2007): 318.
• Organic Chemistry Lab Manual. SC/CHEM 2020.06. Toronto: York University Department of Chemistry, Fall-Winter 2007-2008.
• Wade, L.G. Organic Chemistry. 6th ed. 2006.

More papers on Chromatography Techniques in Organic Chemistry:

• Essay # 102990 : "Organic Chemistry and Life"
• Essay # 72827 : "Organic Chemistry"
• Analytical Essay # 127678 : "Therapeutic Techniques in Film"

APA Citation:

MLA Citation: