Coliphages

Paper Summary:

This paper explains that virtually all bacteria strains have viruses specific to them. The paper then describes an experiment that takes coliphages from sewage and, after enrichment and filtration processes, exposes the E.Coli R10 strain to the phages to obtain the phage specific to the strain. The paper further explains that the bacteria strains are grown on agar plates in the presence of coliphages, and strains W3104-A on plates K12 and W3104 and R12-B on plate K12 developed resistance. The paper then reports that the W3104-A strain is further tested and retains its resistance.

Table of Contents:
Abstract
Introduction
Materials and Method
Isolation and Enrichment of Coliphage from Raw Sewage
Filtration of Coliphage Mixtures
Dilution and Plaque Preparation of R10A and R10B E. Coli Strains
Mutation and Isolation of Phage-Resistant E. Coli Strains
Sensitivity of Phage-Resistant Mutants to Bacteriophages
Results
Discussion
Table: Colonies of Bacterial Strains Response to Coliphages

From the Paper:

"Bacteria have the ability to develop resistance to viruses [6], so as it can be expected some strains (namely W3104-A and R12-B) developed phage-resistant colonies. It is known that certain genetic elements called "episomes" are instrumental in creating phage-resistance in E. Coli strains such as K12 [8]. As indicated in table 1, there were numerous contaminations; this was certainly due to many of the unfiltered cultures that were used. This was done because of time constraints and shortage or missing supplies in the lab."

Sample of Sources Used:

• Goldstein N. 2004. Viruses. The Rosen Publishing Group.
• Ackermann H.W. and Berthiaume L. 1995. Atlas of Virus Diagrams. CRC Press.
• Dennis R. Burton D.R. 2001. Antibodies in Viral Infection. Springer.
• Barksdale L. and Arden SB. 1974. Persisting bacteriophage infections, lysogeny, and phage conversions. Annu Rev Microbiol: 28(0):265-99.
• Udeh P.J. 2004. A Guide to Healthy Drinking Water: All You Need to Know about the Water You Drink. iUniverse.

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