This work examines the valuable role that stable isotope analysis plays in the reconstruction of past diets. The methods used to isolate and subsequently analyse stable isotopes obtained from skeletonized remains is explored. An in-depth discussion of carbon and nitrogen demonstrates their usefulness in distinguishing individuals who had a diet high in either plants, land-based animals, or marine food. To further illustrate the benefit of isotopic analysis, a case study regarding the adoption of maize in South and North America is discussed.
From the Paper:
"Due to its use in radiocarbon dating, methods of isolating collagen had already been developed before its importance in stable isotope studies was realized (Katzenberg, 2000). There are three common techniques employed in the extraction of collagen from bone. The first method is best utilized on fresh and well-preserved archaeological bone. Initial preparation of the material involves the sample being ground and then soaked in hydrochloric acid (HCl). At this stage, the sample is composed primarily of bone organics and soil contaminants and must be placed in sodium hydroxide (NaOH) (Schwarcz & Schoeninger, 1991). Unfortunately, some collagen is lost when soaked in NaOH but this process is necessary as it has been demonstrated that contaminants may skew 13C values (Katzenberg, 2000). "